Tuesday, January 22, 2013

The Cpx Stress Response Confers Resistance to Some, but Not All Bactericidal Antibiotics.

The Cpx Stress Response Confers Resistance to Some, but Not All Bactericidal Antibiotics.

Jan 2013


Princeton University, Department of Molecular Biology Princeton, NJ 08544.


It has recently been suggested that bactericidal antibiotics including aminoglycosides (AGAs), and toxic small molecules, such as hydroxyurea (HU), kill bacteria the same way; namely, by generating reactive oxygen species (ROS) via a process requiring activation of the Cpx stress response. We suggest an opposite, protective role for Cpx. We have confirmed the initial finding that cpxA null mutations confer resistance to HU. However, the two-component sensor CpxA is both a kinase and a phosphatase, and previous work from our lab has shown that removing CpxA can activate the stress response owing to buildup of the phosphorylated response regulator (CpxR∼P) that occurs in the absence of the phosphatase activity. We show that a dominant cpxA* mutation that constitutively activates the Cpx stress response confers a high level of resistance to both HU and AGAs in a CpxR dependent manner. In contrast, inactivating the CpxR response regulator, by mutating the phosphorylation site (D51A) or the putative DNA-binding motif (M199A) does not increase resistance to HU or AGAs. Taken together, these results demonstrate that activation of the Cpx stress response can protect cells from HU and AGAs. However the Cpx response does not increase resistance to all classes of bactericidal antibiotics, as the cpxA* mutants are not significantly more resistant to fluoroquinolones or β-lactams than wild-type cells. Thus, it seems unlikely that all bactericidalantibiotics kill by the same mechanism.